Ayr's tries came from Stuart Fenwick, Gordon Sykes and a double from Florian Martin.
Pat MacArthur came off the bench for Ayr, the day after helping Glasgow Warriors to a win over Leinster.
Glasgow Hawks and Watsonians fought out a 16-16 draw on monsoon conditions at Old Anniesland.
A Grant Strang try and a conversion and three penalties from Stephen O'Donnell gave Hawks a 16-6 lead at half-time but the Edinburgh men fought back in the second period to share the spoils.
West Of Scotland slumped to a 22-6 defeat to Boroughmuir at Meggetland. West's points came from two penalties by Conor Davis.
Hydro Electric Division One
Boroughmuir 22 - West Of Scotland 6
Edinburgh Acads 0 - Ayr 28
Glasgow Hawks 16 - Watsonians 16
Melrose 21 - Hawick 3
Hydro Electric Division Two
Aberdeen GSFP 34 - Hamilton 13
Biggar 3 - Musselburgh 14
Gala 16 - Kelso 0
Haddington 19 - Peebles 0
Jed-Forest 16 - GHA 11
Stewart's Melville FP 11 - Dundee HSFP 6
Hydro Electric Division Three
Morgan Academy FP 7 - Ardrossan Acads 14