A Commonwealth Youth Games gold medallist in 7s back in 2023, Ventisei is one of eight Glasgow academy prospects selected by Head Coach Kenny Murray, having previously led Scotland U20 in a friendly against Wales last month.
Two of Ventisei's team-mates from the 2023 gold-medal winning squad are also selected by Murray and his coaching team, as Matthew Urwin and Fergus Watson are named amongst the back-line options.
Kerr Yule joins the trio in the backs, after having been involved in both the U20 Six Nations and World Rugby U20 Trophy campaigns last year.
Up front, Ollie McKenna and Jake Shearer are named amongst the options at prop, the duo having both joined Glasgow Warriors' senior academy in the summer of 2024.
Joe Roberts joins them in the front-row, the Aberdonian having impressed in training with the Glasgow Warriors senior squad to date in 2024/25.
Dan Halkon rounds out the Glasgow contingent, the second-rower set for his first experience of the U20 Six Nations after being part of the World Rugby U20 Trophy squad last summer.
The group consists of 19 forwards and 15 backs, and will get their championship underway at home to Italy U20 on Friday 31 January at Hive Stadium.
Scotland U20 squad for 2025 U20 Six Nations
Forwards
Billy Allen - Dungannon RFC
Ollie Blyth-Lafferty - Edinburgh Rugby
Freddy Douglas - Edinburgh Rugby
Oliver Duncan - Edinburgh Rugby
Oliver Finlayson-Russell - St Andrews University
Mark Fyffe - Edinburgh University
Bart Godsell - Loughborough University
Dan Halkon - Glasgow Warriors
Christian Lindsay - Loretto School/Edinburgh Rugby
Reuben Logan - Northampton Saints
Ollie McKenna - Glasgow Warriors
Charlie Moss - Montpellier
Will Pearce - Cardiff Metropolitan University/Bristol Bears
Joe Roberts - Glasgow Warriors
Jake Shearer - Glasgow Warriors
Pat Spence - Heriot's RFC
Seb Stephen - Edinburgh Academical Football Club
Jamie Stewart - Edinburgh Rugby
Ryan Whitefield - Biggar RFC
Backs
Jack Brown - Edinburgh Rugby
Isaac Coates - Edinburgh Rugby
Noah Cowan - Brunel University/Ealing Trailfinders
Angus Hunter - Heriot's RFC
Hamish MacArthur - Edinburgh Rugby
Nairn Moncrieff - Edinburgh Rugby
Hector Patterson - Edinburgh Rugby
Guy Rogers - Surrey University/Harlequins
Matthew Urwin - Glasgow Warriors
Cameron van Wyk - Ayr RFC
Johnny Ventisei - Captain - Glasgow Warriors
Fergus Watson - Glasgow Warriors
Campbell Waugh - Glasgow Hawks
Ross Wolfenden - Edinburgh Rugby
Kerr Yule - Glasgow Warriors